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. Author manuscript; available in PMC: 2024 Oct 24.
Published in final edited form as: Brain Behav Immun. 2023 Dec 22;116:269–285. doi: 10.1016/j.bbi.2023.12.025

Fig. 4.

Fig. 4.

Conditional ablation of microglial TNFR2 has a sex-specific impact on microglial and leukocyte populations after IS and SCI. (A) Flow cytometry plots showing gating strategies for microglia and leukocyte analysis in the ipsilateral cortex at day 5 post-pMCAO. (B-C) Flow cytometry analysis of infiltrating immune cell populations and microglia in the ipsilateral cortex of female (B) and male (C) Cx3cr1CreER:Tnfrsf1bfl/fl mice and Tnfrsf1bfl/fl littermates at day 5 post-pMCAO (n = 5–6/group females, n = 5–7/group males). * p < 0.05, Student’s t-test. (D) Flow cytometry plots showing gating strategies for microglia and leukocyte analysis in the spinal cord at day 7 post-SCI. (E) Flow cytometry analysis of infiltrating immune cell populations and microglia in the spinal cord of female Cx3cr1CreER:Tnfrsf1bfl/fl mice and Tnfrsf1bfl/fl littermates at day 7 post-SCI (n = 3–4/group). * p < 0.05, ** p < 0.01; Student’s t-test.