. 2024 Oct 23;14:25058. doi: 10.1038/s41598-024-74830-6

Table 4.

Experimental design RSM based on CCD with actual and predicted response for EPS production.

Std.	Run	Point type	Carbon source g L⁻¹	Nitrogen source g L⁻¹	pH	Bacterial concentration ml L⁻¹	EPS g L⁻¹
Std.	Run	Point type	Carbon source g L⁻¹	Nitrogen source g L⁻¹	pH	Bacterial concentration ml L⁻¹	Actual	Predict
26	1	Center	30	30	8	30	3.1	3.15
5	2	Fact	20	20	9	20	1.67	1.89
29	3	Center	30	30	8	30	3.28	3.15
27	4	Center	30	30	8	30	3.26	3.15
22	5	Axial	30	30	10	30	0	0.2
9	6	Fact	20	20	7	40	6.5	6.3
24	7	Axial	30	30	8	50	3.64	3.54
28	8	Center	30	30	8	30	3	3.15
15	9	Fact	20	40	9	40	1.12	1.04
30	10	Center	30	30	8	30	3.19	3.15
3	11	Fact	20	40	7	20	3.42	3.52
19	12	Axial	30	10	8	30	3.19	3.09
23	13	Axial	30	30	8	10	3.24	3.16
21	14	Axial	30	30	6	30	6.83	6.85
1	15	Fact	20	20	7	20	6.5	6.45
18	16	Axial	50	30	8	30	2.81	2.72
17	17	Axial	10	30	8	30	3.67	3.58
14	18	Fact	40	20	9	40	0.81	0.77
11	19	Fact	20	40	7	40	3.6	3.82
20	20	Axial	30	50	8	30	1.1	1.04
25	21	Center	30	30	8	30	3.09	3.15
7	22	Fact	20	40	9	20	1	0.91
16	23	Fact	40	40	9	40	1.5	1.67
10	24	Fact	40	20	7	40	5	5.21
4	25	Fact	40	40	7	20	3.6	3.46
8	26	Fact	40	40	9	20	0.86	1.13
6	27	Fact	40	20	9	20	0.79	0.68
2	28	Fact	40	20	7	20	4.8	4.95
13	29	Fact	20	20	9	40	1.32	1.57
12	30	Fact	40	40	7	40	4.32	4.17