TABLE 1.

Sense primers used to introduce site-directed mutations into clone pMVE-1-51^a

Primer name	Sequenceb	Amino acid mutation
E-277-a-fwd	5′-CAGTCGAGTTTTCGAGCATCACACTTAAACTCACTTCAG-3′	E-277 Ser→Ile
E-277-b-fwd	5′-CAGTCGAGTTTTCGAGCAACACACTTAAACTCACTTCAG-3′	E-277 Ser→Asn
E-277-c-fwd	5′-CAGTCGAGTTTTCGAGCGTCACACTTAAACTCACTTCAG-3′	E-277 Ser→Val
E-277-d-fwd	5′-CAGTCGAGTTTTCGAGCCCCACACTTAAACTCACTTCAG-3′	E-277 Ser→Pro
E-390-a-fwd	5′-TTGTGGTAGGCAGGGGAGAGAAGCAGATCAATCACCACT-3′	E-390 Asp→Glu
E-390-b-fwd	5′-TTGTGGTAGGCAGGGGAAACAAGCAGATCAATCACCACT-3′	E-390 Asp→Asn
E-390-c-fwd	5′-TTGTGGTAGGCAGGGGATACAAGCAGATCAATCACCACT-3′	E-390 Asp→Tyr

^aAntisense complementary primers (not shown) were used in combination with sense primers to form primer pairs for site-directed mutagenesis. 

^bNucleotide substitutions are underlined and affected codons are highlighted in bold.