TABLE 2.

Phenotypes of hinge and RGD mutant viruses in vitro

Virus	Mutation	Plaque phenotypea	Temp sensitivity at 39°C	Virus entryb
				Absorption		Penetration
				30:90	60:90	30:90	60:90
CDV-1-51	None	Normal	None	46	73	47	78
CDV-1-51v1	E-277 (S→I)	Normal	None	43	72	49	75
CDV-1-51v2	E-277 (S→N)	Large	None	48	81	47	82
CDV-1-51v3	E-277 (S→V)	Normal	None	49	79	51	80
CDV-1-51v4	E-277 (S→P)	Normal	None	44	77	49	80
CDV-1-51v5	E-390 (D→N)	Small	None	46	74	50	77
CDV-1-51v6	E-390 (D→E)	Large	None	50	80	51	82
CDV-1-51v7	E-390 (D→Y)	Small	None	44	74	43	77

^aPlaque phenotypes were determined by comparison to those observed for CDV-1-51. CDV-1-51 had a normal plaque phenotype, with a diametric size of approximately 2.2 mm at day 5 p.i. Viruses with small-plaque phenotypes had plaques approximately half this size at the same time p.i. Viruses with large-plaque phenotypes had plaques approximately twice the size of normal plaques at the same time p.i. 

^bVirus entry rates (ratios) for adsorption and penetration assays are expressed as a percentage, i.e., the number of plaques observed at 30 (30:90) or 60 (60:90) min p.i. divided by the number observed at 90 min p.i. (times 100%).