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Effect of CAR expression on adenovirus-mediated gene transfer to NIH 3T3 cells. Cells were infected with varying MOIs of Ad-CaPi coprecipitates encoding wt-CAR (◊). Tailless-CAR (○), GPI-CAR (▵), or CFTR (□) as a control. One day later cells were infected with varying MOIs of Ad2/GFP. Data are the percentage of GFP-positive cells for cells infected with the Ad/CAR vectors at MOIs of 50 (A), 20 (B), 6 (C), and 1 (D).
