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. 2001 Aug;75(16):7703–7711. doi: 10.1128/JVI.75.16.7703-7711.2001

FIG. 6.

FIG. 6

FIG. 6

Effect of neuraminidase or glycosidase treatment on adenovirus-mediated gene transfer to CAR expressing airway epithelia. (A) Two days after CAR gene transfer, airway epithelia were pretreated with neuraminidase (Neur), O-glycosidase (O-gly), or N-glycosidase (N-gly) and stained with FITC-labeled WGA. Data are en face projections of WGA binding to the apical membrane of airway epithelia. WGA binding (in relative intensity units) decreased following treatment with neuraminidase (14.5 ± 1.9), O-glycosidase (21.0 ± 1.4), and N-glycosidase (20.3 ± 1.2) compared to control (29.5 ± 1.0) (n = 9 for each). (B) Treated epithelia were also assayed for gene transfer with Ad2/βGal. Fluorescence and β-Gal data are means + standard errors of the means (error bars) (n = 6).