Figure 4.
Bifidobacterium species are resistant to high levels of chlorine. Chlorine susceptibility was determined using Kirby-Bauer disk diffusion method.19 Zone of inhibition was measured as the distance between chlorine disk and edge of bacterial growth. All microbes were grown on TSA broth and cultured in anaerobic conditions for 48 hours at 37°C. Bars represent mean ± SEM with dots representing a biological replicate. One-way ANOVA with multiple comparisons for each species; *P < 0·05; **P < 0·01; ***P < 0·001; ****P < 0·0001.