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. Author manuscript; available in PMC: 2024 Oct 24.
Published in final edited form as: Nat Methods. 2024 Jun 5;21(6):1094–1102. doi: 10.1038/s41592-024-02275-w

Extended Data Fig. 1. Principle and schematic of TICO microscope.

Extended Data Fig. 1

(a-c) Design principle of TICO microscope: (a) conventional strategy for incorporation a DMD into a confocal microscope; (b) bypassing DMD in the detection path to avoid fluorescence loss; (c) inserting a wedge prism in front of the DMD corrects for both image plane tilt and 1D magnification change caused by the DMD, restoring confocality between excitation and detection beams. (d) Detailed schematic of TICO microscope. DM, dichromatic mirror. Em, emission filter. Ex, excitation filter. PBS, polarizing beam splitter. λ/2, half-wave plate. λ/4, quarter-wave plate. Galvo, galvanometric scanner. DMD, digital micromirror device. Obj, objective.