(a) Confocal image of Voltron2 fluorescence. Scale bar, 50 μm. (b) Averaged Voltron2 fluorescence image with 14 neurons targeted within the FOV. Scale bar, 50 μm. (c) Voltron fluorescence traces of 5 active neurons over a 10 s recording. Recording speed 2 kHz. (d,e) Zoomed-in fluorescence traces over the rectangular labeled regions in (c). (f) Confocal image of GFP fluorescence. Scale bar, 20 μm. (g) Averaged somArchon fluorescence image with 4 neurons targeted within the FOV. Scale bar, 20 μm. (h) SomArchon fluorescence traces of 2 active neurons over a 10 s recording. Recording speed 4 kHz. (i,j) Zoomed-in fluorescence traces over the rectangular labeled regions in (h).