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. 2024 Jul 18;22(11):3037–3050. doi: 10.1111/pbi.14426

Figure 1.

Figure 1

Analysis of cold tolerance in TIP2;1, TIP3;2 and TIP4;3‐overexpressing transgenic plants. (a–c) Cold‐tolerance phenotypes (a), relative TIP2;1 expression levels (b), and leaf injury area (c) of wild‐type (WT) and TIP2;1‐overexpressing transgenic plants (OE#5, OE#7) after cold treatment. (d–f) Cold‐tolerance phenotypes (d), relative TIP3;2 expression levels (e) and leaf injury area (f) of WT and TIP3;2‐overexpressing transgenic plants (OE#4, OE#5) after cold treatment. (g–i) Cold‐tolerance phenotypes (g), relative TIP4;3 expression levels (h), and leaf injury area (i) of WT and TIP4;3‐overexpressing transgenic plants (OE#2, OE#8) after cold treatment. In (a, d, g), 14‐day‐old seedlings grown at 25 °C were incubated at 4 °C for 2 days. Representative photographs were taken after 2 days of recovery at 25 °C. In (b, c, e, f, h, i), Error bars represent mean ± SD (standard deviation). *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001 (two‐sided t‐test).