TABLE 1.
Cooperation of EBNA3C with Rasa
| No. of colonies/dish in expt no.:
|
||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| 1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 | 11 | 12 | 13 | 14 | |
| Plasmid | ||||||||||||||
| pcDNA3 | 0 | 4 | 3 | 2 | 0 | 2 | 2 | 2 | 0 | 0 | 0 | 1 | 4 | 0 |
| WT3C | 13 | 13 | 20 | 11 | 9 | 14 | 12 | 9 | 13 | 7 | 9 | 18 | 9 | 11 |
| ΔPLDLS | 2 | 5 | 5 | 3 | 5 | 6 | 6 | 4 | 6 | 4 | 0 | 5 | 5 | 2 |
| ALDAS | 0 | 1 | 6 | 5 | 4 | 4 | 3 | 1 | 1 | 2 | 0 | 0 | 3 | 3 |
a
In each assay, 15 μg of EBNA3C expression plasmid or the pcDNA3 vector was cotransfected with 5 μg of EJ6.6 (Ha-Ras expression vector). Cells were then grown for 21 to 28 days with G418 (400 μg/ml). The highly refractile, morphologically transformed colonies were then counted under a microscope.