TABLE 2.

BVDV DI9c ORF derivatives

Mutant	Mutation type and positiona	No. of inserted or deleted residues
NS3
	Substitutions in the DEYH box [1919–1922]
1ab	[1919] D→L
1bb	[1921] Y→T
1cb	[1921] Y→F
1db	[1921] Y→E
	Insertions
2b	[1693] RV	2
3b	[1834] TRQL	4
4b	[1966] LTRQ	4
5b	[2088] NTRV	4
8b	[2099] H	1
7b	[2191] DRRV	4
8b	[2249] DRRV	4
	Deletion
9b	[2090–2099]	10
	Substitutions for S1761 (serine protease)
10ac	[1761] S→A
10b	[1761] S→T
10c	[1761] S→C
NS4A
	Insertions
11	[2309] DASI	4
NS4B
	Insertions
12	[2361] HACA	4
13	[2377] TRRS	4
14	[2456] ETRL	4
15	[2668] HA	2
NS5A
	Insertions
16	[2776] TRVC	4
17	[2955] TR	2
18	[3065] RRVD	4
19	[3114] TRVT	4
	Deletions
20	[2738–2776]	39
21	[2776–2955]	180
NS5B
	Insertions
22	[3259] TRGA	4
23	[3352] YAWS	4
24	[3379] DASI	4
25	[3545] RRVD	4
	Deletion
26	[3428]	1
	Substitutions in motif C [3635–3637]
27d	[3636]D→T + [3637]D→R
Cleavage sites
	Substitutions at P1 and/or P1′ position
28	[2281] L→R(P1)
29	[2345] L→R(P1)
30	[2692] L→R(P1)
31	[3188] L→D(P1) + [3189] S→P(P1′)

^aThe numbering scheme refers to that of Fig. 1. Numbers in brackets correspond to the affected positions of the BVDV CP7 nonstructural polyprotein (26). Substitution and deletion mutations are denoted by the affected amino acid(s); insertions mutations are denoted by the preceding amino acid of the BVDV CP7 polyprotein. The number of inserted residues is indicated. 

^bConstruction described in reference 12. 

^cConstruction described in reference 4. 

^dConstruction described in reference 37.