FIGURE 3.

Gene expression and activity of glycocalyx‐degrading enzymes in lungs. Real‐time RT PCR was performed to determine the baseline mRNA expression levels of (a) matrix metalloproteinase (MMP) 2 and (b) MMP14 in lung tissue of WT and HPSE ^−/− mice. (c) HPSE activity and (d) MMP14 activity were determined in the lung tissue after I/R injury and in uninjured tissue and was normalized to the normal plasma level in each genotype WT or HPSE ^−/− to calculate the fold change. (e) Gelatin zymography was performed to visualize MMP2 and MMP9 activity in lung tissue after I/R injury. *p < 0.05, **p < 0.01, ***p < 0.001.