Figure 6.

PKD1 in myeloid lineage cells contributes to the expression of MHC-II in neutrophils and macrophages infiltrated into the bronchial space and lung interstitium following repeated exposures to S. rectivirgula. WT (n = 5 to 6/group) or PKD1mKO mice (n = 5/group) were exposed intranasally to S. rectivirgula (100 μg) three times per week for 3 weeks. BAL cells (A) and LICs (B) were isolated from mice at 48 h after the last S. rectivirgula exposure. Cells were stained with fluorochrome-conjugated Abs and then analyzed by flow cytometry and FlowJo flow software. Gating strategies for flow cytometric analysis of BAL cells and LICs are shown in Supplementary Figures 3A, B , respectively. Levels of surface expression of MHC-II on cells were determined by geometric mean fluorescent intensity (gMFI) of each marker in the indicated cell population. Each dot or square represents individual mouse. Data represent the mean gMFI ± SD. Significance was determined by two-tailed Student’s t-test. Statistically significant differences are indicated (*p < 0.05; **p < 0.01). ns, not significant.