FIG. 3.

Effect of sodium chlorate on dengue virus-binding molecules. (A) Effect of low-sulfate medium and 50 mM sodium chlorate on dengue virus infection of Vero cells. Florescent focus units in untreated and treated cells were determined. The number of fluorescent focus units obtained with untreated cells was taken as 100%. For the procedure, see Materials and Methods. (B) Membrane proteins extracted from Vero cells grown in standard medium (lane 1) or in low-sulfate DMEM in the presence of 10 and 50 mM sodium chlorate (lanes 2 and 3, respectively) were subjected to SDS–10% PAGE, transferred to nitrocellulose membranes, and incubated with 100,000 cpm of [³⁵S]methionine-labeled dengue virus. Molecular size markers are indicated on the left.