Effects of constitutive IκBα-ΔN expression on antiflavivirus capability of salicylates. The ability of JEV (A) or DEN (B) to replicate in IκBα-ΔN-expressing cell clones (IκB#1, #8, #12, #17, and #18) was compared to that in wild-type BHK-21 cells by measurement of titers of virus released into the culture medium. (C) The anti-JEV effects of salicylates were determined for the IκBα-ΔN-expressing cell clones (IκB#12 and #17) and the wild-type BHK-21 cells. After 1 h of JEV infection, cells were left untreated or treated with 5 mM NaSal (SA) or aspirin (ASA) and then incubated at 37°C for another 24 h. Virus production in the culture medium was determined by a plaque assay. Data are mean virus titers for two independent experiments.