FIG. 8.

Antiflavivirus capability of salicylates in cells treated with the p38 MAPK inhibitor SB203580. (A) BHK-21 cells were infected by JEV at an MOI of 5, and at 1 h postinfection SB203580 (SB) was added to the cells at varying micromolar concentrations in the presence or absence (−) of 5 mM NaSal (SA). After 36 h of incubation at 37°C, virus yields in the culture media were determined by a plaque assay. (B) BHK-21 cells were infected by DEN at an MOI of 5, and at 1 h postinfection varying doses (micromolar concentrations) of SB203580 or its ineffective analogue SB202474 (SBa) were added cells in the presence or absence (−) of 5 mM SA. After 36 h of incubation at 37°C, virus yields in the culture media were determined as described for Fig. 1A.