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. 2024 Feb 17;202(12):5531–5544. doi: 10.1007/s12011-024-04093-9

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© The Author(s) 2024

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 6 — SiNrf2 exacerbates ferroptosis induced by glucolipotoxicity in MIN6 cells. A Nrf2 protein expression detected and quantified using western blot. B Cell viability measured in MIN6 cells after silencing Nrf2 using the CCK8 assay. C–E GSH, SOD, and MDA levels determined in MIN6 cells after silencing Nrf2 genes. F, G Lillie staining was used to detect and quantify ferrous iron content (blue) in MIN6 cells after silencing Nrf2 genes (× 400). H–J XCT and GPX4 protein expression levels detected and quantified using western blot analysis. Data are expressed as mean ± SD (*P < 0.05 vs. Control; #P < 0.05 vs. GP; &P < 0.05 vs. siNrf2 + GP)