Fig. 1. Film-like organelles for selective RNA modification.

Cartoon showing a film-like organelle mounted on the plasma membrane. The colored shading indicates the organelle microenvironment, RNA is recruited through ms2 loops into the organelle, and modified stop codons result in the full-length production of protein. gRNAs are not shown to keep the illustration simple. DKC1 is active as a tetrameric complex with NOP10, NHP2, and GAR1, which are also drawn here for reference. Our method enabled us to utilize the endogenous pool of those, which alleviated the need to engineer any of those.