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. 2001 Sep;75(17):7848–7853. doi: 10.1128/JVI.75.17.7848-7853.2001

FIG. 2.

FIG. 2

Sucrose gradient sedimentation of GST-COPV L1 and COPV L1 proteins. GST-COPV L1 or COPV L1 was analyzed by sedimentation ultracentrifugation on a 5 to 30% sucrose gradient. The fractions were assayed for GST-COPV L1 (A) or COPV L1 (B) by immunoblotting with anti-AU1 monoclonal antibody. The amount of GST-COPV L1 in the fractions was determined by densitometry and plotted as the percentage of total GST-COPV L1 protein (C). Hemoglobin (4.5S), catalase (11S), and β-galactosidase (19S) were used as sedimentation markers.