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. 2024 Oct 25;10(1):96. doi: 10.1038/s41537-024-00498-6

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© The Author(s) 2024

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 4 — a ICC of MAP2 in differentiated cells, THC-treated cells, and undifferentiated control cells. On the left side, sole MAP2-staining (green) is presented, whereas, on the right side, the corresponding merge pictures of the co-staining with TUBB3 (βIII-Tubulin) (red) and nuclei (blue) are depicted. The scale bar represents 100 µm. b ICC of DRD2 (left panel) and CNR1 (right panel) in differentiated cells, THC-treated cells and undifferentiated control cells. In the respective left column sole DRD2- and CNR1-, staining (green) is presented, whereas, in the right column, the corresponding merge pictures of the co-staining with TUBB3 (βIII-Tubulin) (red) and nuclei (blue) are depicted. Unfortunately, in the case of the DRD2 staining TUBB3 has not been stained for practical reasons. Scale bar: 100 µm. c ICC of Grik (left panel) and GRIN1 (NMDAR1) (right panel) in differentiated cells, THC-treated cells, and undifferentiated control cells. In the respective left column sole Grik- and GRIN-, respectively, staining (green) is presented, whereas, in the right column, the corresponding merge pictures of the co-staining with TUBB3 (βIII-Tubulin) (red) and nuclei (blue) is depicted. The scale bar represents 100 µm. d ICC of SYP (left panel) and DLG4 (PSD95) (right panel) in differentiated cells, THC-treated cells, and undifferentiated control cells. In the respective left column, sole SYP- and DLG4-staining (green) is presented, whereas, in the right column, the corresponding merge pictures of the co-staining with βIII-Tubulin (red) and nuclei (blue) is depicted. The Scale bar represents 100 µm.

Fig. 4 — a ICC of MAP2 in differentiated cells, THC-treated cells, and undifferentiated control cells. On the left side, sole MAP2-staining (green) is presented, whereas, on the right side, the corresponding merge pictures of the co-staining with TUBB3 (βIII-Tubulin) (red) and nuclei (blue) are depicted. The scale bar represents 100 µm. b ICC of DRD2 (left panel) and CNR1 (right panel) in differentiated cells, THC-treated cells and undifferentiated control cells. In the respective left column sole DRD2- and CNR1-, staining (green) is presented, whereas, in the right column, the corresponding merge pictures of the co-staining with TUBB3 (βIII-Tubulin) (red) and nuclei (blue) are depicted. Unfortunately, in the case of the DRD2 staining TUBB3 has not been stained for practical reasons. Scale bar: 100 µm. c ICC of Grik (left panel) and GRIN1 (NMDAR1) (right panel) in differentiated cells, THC-treated cells, and undifferentiated control cells. In the respective left column sole Grik- and GRIN-, respectively, staining (green) is presented, whereas, in the right column, the corresponding merge pictures of the co-staining with TUBB3 (βIII-Tubulin) (red) and nuclei (blue) is depicted. The scale bar represents 100 µm. d ICC of SYP (left panel) and DLG4 (PSD95) (right panel) in differentiated cells, THC-treated cells, and undifferentiated control cells. In the respective left column, sole SYP- and DLG4-staining (green) is presented, whereas, in the right column, the corresponding merge pictures of the co-staining with βIII-Tubulin (red) and nuclei (blue) is depicted. The Scale bar represents 100 µm.