Fig. 5. Western blot analysis: mean x-fold expression (±SD) of TUBB3 (βIII-Tubulin), DRD2, Grik, GRIN (NMDAR1), SYP and DLG4 (PSD95) in undifferentiated control cells, under application of 50 µM retinoic acid (RA50) in combination with THC20 in relation to expression level in solely RA50-treated SH-SY5Y cells (=1).

There were no significant differences in expression between differentiated cells and differentiated cells that were additionally treated with Cannabinoids. However, in trend, cannabinoid treatment led to an approximation of expression toward the expression level in untreated/undifferentiated control cells in the investigated targets. Representative bands are shown over each column for each target and condition. N-numbers of western blot experiments for the different targets were: TUBB3: 3, DRD2: 2, Grik: 2, NMDAR: 2, SYP: 2, DLG4: 3.