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. 2005 Jun 13;102(25):8922–8926. doi: 10.1073/pnas.0503840102

Fig. 2.

Fig. 2.

Oocytes from Gpr3–/– mice display age-independent premature resumption of meiosis I, associated with increase in morphological abnormalities. (A) Antral oocytes from 3.5-week (3.5w)- and 6-month (6m)-old ovaries were collected and scored for presence of a GV, GV breakdown (GVB), or extrusion of first polar body (PB) at collection time (d1) and after 18 h in culture (d2). The proportion of oocytes undergoing parthenogenetic division (2c), lysis (L), or fragmentation (FGT) was also scored. For both ages, a clear decrease in the proportion of GV stage was observed in Gpr3–/– oocytes (P < 0.001) at collection time (d1); spontaneous resumption of meiosis was impaired after 18 h in culture (d2). (B) Meiotic competence expressed as percentage of GV stage oocytes having extruded the first polar body after 18 h in culture. For A and B, open columns, Gpr3+/+ and black columns, Gpr3–/– animals. (C) Representative morphology of oocytes observed in light microscopy by using Nomarski optics. (a) Oocyte with GV intact, (b) oocyte showing GV breakdown, (c) oocyte with first polar body extruded, (d) oocyte with two polar bodies extruded, (e) lysed oocyte, and (f) fragmented oocyte. (Bar, 10 μm.)