Table 1.
A listing of all detected ion signals of DNA fragments and E9 DNase–DNA complexes, their molecular masses and assignments
| Abbreviation | Ions (m/z) | Mw (Da) | |
|---|---|---|---|
| ssDNA fragments | |||
| GTAAAACGACGGCCAGT | 5′-17-3′ | 1308.2 (4+), 1743.9 (3+) | 5228.5 |
| GTAAAACGACGGCC | 5′-14 | 1071.5 (4+), 1428.3 (3+) | 4281.9 |
| GTAAAACGACGG | 5′-12 | 1235.7 (3+) | 3703.5 |
| GTAAAACGAC | 5′-10 | 1016.1 (3+) | 3045.1 |
| GTAAAAC | 5′-7 | 1057.8 (2+) | 2113.6 |
| GTAAAA | 5′-6 | 913.1 (2+) | 1824.3 |
| GTAAA | 5′-5 | 756.5 (2+) | 1511.0 |
| GTAA | 5′-4 | 599.8 (2+) | 1197.6 |
| AACGACGGCCAGT | 13-3′ | 1013.3 (4+), 1350.5 (3+) | 4048.6 |
| ACGACGGCCAGT | 12-3′ | 1246.2 (3+) | 3735.6 |
| CGACGGCCAGT | 11-3′ | 1141.7 (3+) | 3422.1 |
| GACGGCCAGT | 10-3′ | 1045.4 (3+) | 3133.2 |
| GGCCAGT | 7-3′ | 734.7 (2+) | 2201.4 |
| CCAGT | 5-3′ | 772.5 (2+) | 1543.0 |
| AGT | 3-3′ | 483.2 (2+), 965.3 (1+) | 964.6 |
| E9:DNase–Ni2+–ssDNA complexes | |||
| E9:DNase-Ni2+-5′-17-3′ | 2548.1 (8+), 2265.1 (9+) | 20 375.6 | |
| E9:DNase-Ni2+-14-3′ | 2440.0 (8+), 2169.0 (9+) | 19 512.6 | |
| E9:DNase-Ni2+-13-3′ | 2400.9 (8+), 2134.3 (9+) | 19 199.8 | |
| E9:DNase-Ni2+-12-3′ | 2361.5 (8+), 2099.2 (9+) | 18 886.1 | |
| E9:DNase-Ni2+-11-3′ | 2322.6 (8+), 2064.6 (9+) | 18 572.8 | |
DNA fragments and non-covalent E9 DNase–Ni2+–ssDNA complexes resulting from the enzymatic reaction. E9 DNase (10 μM) was incubated with Ni2+ (20 μM) and 17mer ssDNA (100 μM) at 20°C for 45 min.