Fig. 2. Rh Bri2 BRICHOS R221E accumulates in the brains of App^NL-G-F and WT mice after intravenous injections.

Representative images of sections from indicated brain regions (column A, DG and B, CA2 and CA3 hippocampal regions; C, RSC, D, auditory and E, olfactory cortex regions) of rh Bri2 BRICHOS R221E- or vehicle (veh)-treated App^NL-G-F (App) and WT mice stained with anti-Bri2 BRICHOS antibody. The areas marked with boxes are shown in the panels below them. The punctuate pattern of staining indicates that rh Bri2 BRICHOS R221E is present intracellularly, see text for details. The bar charts show the rh Bri2 BRICHOS-positive area (% of total area) in the different brain regions for each treatment group. Scale bars represent 400 μm and apply to all panels in the respective column, except the magnified areas for which the scale bars are 100 μm. Data are shown as mean ± SEM (n = 6–7 mice per group). One-way ANOVA test and Tukey’s test were used to calculate the p-values. ^∗p < 0.05, ^∗∗p < 0.01, ^∗∗∗∗p < 0.0001. Auditory auditory cortex, CA Cornu ammunis, DG dentate gyrus, Olfactory olfactory cortex, RSC retrosplenial cortex.