Fig. 5. Multiple cilia in beta cells.

a Expanded multiple islet cell cilia of one mouse pancreas stained by acetylated tubulin (magenta) and Arl13b (yellow). Scale bar: 1 µm. b Single FIB-SEM slices of one mouse pancreatic islet showing a beta cell containing two primary cilia sharing one ciliary pocket. The membrane is outlined in purple. Z-distances from the first image are indicated in the upper right of the following slices. Scale bar: 500 nm. c FIB-SEM data of one mouse pancreatic islet with a beta cell containing two primary cilia (C1 and C2) with distinct ciliary pockets. The cilia are pointing in opposite directions. The raw FIB-SEM slice shows the two cilia with basal bodies (BB) and membranes outlined in purple. Scale bar: 1 µm. The 3D rendering shows the plasma membrane in purple and the cilia microtubule structures in gray. The rendering on the right shows basal bodies (BB), daughter centrioles (DC), and axonemes (Ax) in gray.