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. 2024 Oct 24;15:9168. doi: 10.1038/s41467-024-53348-5

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© The Author(s) 2024

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 7 — a Single tomographic slices of primary cilia in human islets from one donor. There are 3 cilia originating in a beta cell with the basal body of one cilium (1) and the axonemes of 2 other cilia visible in the slice (2 and 3, purple). The neighboring alpha cell is outlined in orange. In magnified views of distant slices through the tomogram (a’ and a”) the end of cilium 2 is visible protruding and extending into a neighboring alpha cell (orange). Axonemes, basal bodies, and centrioles are rendered in gray. Scale bars: 500 nm and 100 nm. b Single tomographic slices of mouse beta cells of one isolated mouse pancreatic islet with 2 primary cilia (purple and magenta) originating from a beta cell (purple) and another islet cell (magenta) meeting in the space between cells. The side view shows the axonemes of the cilia in close proximity. The 3D view shows the segmentation of ciliary and plasma membranes together with the axonemes. Scale bars: 200 nm. c Tomographic slices of a ssET volume of one mouse pancreatic islet showing a large ciliary pocket shared by a primary cilium originating from a beta cell (purple) and a primary cilium of unknown origin (yellow). The 3D rendering shows the interaction of the two cilia. Axonemes are in gray. Scale bars: 100 nm.