Figure 1.

Flow cytometric analysis of C1498 cells (A and C) and germ cells (B and D) from normal C57BL/6 mice. Gates for FACS were set for FSC and SSC (A and B). G1 comprises the FSC^high and SSC^low fractions. The staining profiles of CD45 compared with H-2K^b/H-2D^b for the G1 cell fraction of C1498 cells (C) and germ cells (D) from normal C57BL/6 mice are shown with quadrant statistics. None of the C1498 cells in the FSC^high and SSC^low fractions were in the CD45^– and H-2K^b/H-2D^b– fraction, whereas 93.5% of the germ cells were in the CD45^– and H-2K^b/H-2D^b– fraction.