Table 7.
Summary of serum cytokines measured following LPS administration (0.25 µg/kg body weight) in cattle supplemented with methionine at 0 (L0), 2.2 (L1), or 4.4 (L2) g/steer/d for 40 d
| Treatment | P-value | ||||||
|---|---|---|---|---|---|---|---|
| Cytokine, pg/mL | L0 | L1 | L2 | SEM | Treatment | Time | Interaction |
| Tumor necrosis factor-α (TNF-α) | 214.0 | 198.0 | 260.9 | 92.0 | 0.87 | <0.01 | 0.03 |
| Interferon-γ (IFN-γ) | 69.5 | 169.1 | 299.4 | 99.5 | 0.26 | 0.01 | 0.12 |
| Interferon-α A (IFN-α A) | 18.5 | 26.4 | 20.2 | 4.0 | 0.37 | <0.01 | 0.06 |
| Interleukin-13 (IL-13) | 68.5 | 140.0 | 198.3 | 61.1 | 0.32 | <0.01 | 0.06 |
| Interleukin-1α (IL-1α) | 9.6 | 22.5 | 34.1 | 11.5 | 0.32 | <0.01 | <0.01 |
| Interluekin-1 F5 (IL-1 F5) | 70.1 | 115.6 | 119.0 | 28.9 | 0.41 | <0.01 | 0.40 |
| Interleukin-21 (IL-21) | 850.2 | 1513.8 | 1191.0 | 334.0 | 0.37 | <0.01 | 0.02 |
| Interferon-γ-inducible protein-1- (IP-10; CXCL10) | 515.1 | 447.3 | 488.1 | 29.6 | 0.26 | <0.01 | <0.01 |
| Monokine induced by Interferon-γ (MIG; CXCL9) | 529.1 | 561.2 | 957.7 | 220.9 | 0.30 | <0.01 | <0.01 |
| Macrophage inflammatory protein-1β (MIP-1β; CCL4) | 266.3a | 206.1b | 217.4b | 14.4 | 0.01 | <0.01 | <0.01 |
a,bMeans within rows lacking common superscripts differ P ≤ 0.05