Figure 5.

Human anti-MUC1 mAbs mediate antibody-dependent cellular trogocytosis. Jurkat MUC1 22TR^hi (A) and Raji MUC1 22TR (B) target cells labeled with calcein-red and DiO and coincubated with CellTrace Violet-labeled healthy donor neutrophils stimulated overnight with IFNγ and G-CSF, at an E:T ratio of 5:1 for 4 h with and without antibodies. Antibody-dependent cellular trogocytosis was measured by taking the %DiO+Calcein−CellTrace+ neutrophils and subtracting the same percentage in wells with no primary antibodies, representing the background trogocytosis. Each dot shows the average of duplicates from n = 3 independent experiments. Bars depict the mean ± SEM. Statistical comparisons were made through one-way ANOVA with Dunnett’s test to correct for multiple comparisons; *** p < 0.001.