Figure 8.

Amount of antibody bound does not correlate with efficiency of ADCC or ADCP but does with ADCT. Cells were stained with 10 µg/mL of each primary antibody for 15 min prior to (A) 4 h incubation with human NK cells that had been stimulated overnight with IL-2 at effector to target ratios ranging from 10:1 to 20:1, (B) 1 h incubation with human THP-1 cells at a 1:1 ratio, or (C) 4 h incubation with human neutrophils stimulated overnight with hIFNγ and G-CSF. Jurkat cells transduced with human CD20 were used with rituximab as positive controls. %ADCC, ADCP, and trogocytosis were calculated as in Figure 3, Figure 4 and Figure 5. Gray error bars represent mean ± SEM for average relative MFI and % antibody-mediated function. There is no significant correlation between the amount of antibody bound (average relative MFI) and average %ADCC (r² = 0.07, ns 10:1; r² = 0.05, ns 20:1) or %ADCP (r² = 0.06, ns), but there is for trogocytosis (r² = 0.81, p < 0.0001).