FIG. 2.

Immunoblots of ICP0 in two-dimensionally separated HSV-1-infected HEL fibroblast cell lysates. HSV-1-infected cells were harvested at 4, 8, and 12 h postinfection (P.I.). In addition cells were also treated with MG132 or roscovitine. MG132 was given to HSV-1-infected cells 8 h after infection and harvested at 12 h. Roscovitine was added to HSV-1-infected cells at 5 h after infection, and cells were harvested at 12 h postinfection. Immunoblots were reacted with ICPO monoclonal antibody.