Figure 3.

Changes in relative abundance of major biotransformation species for four ADC in mouse preclinical studies as a function of time postdose. (A) Thio-succinimide hydrolysis species. LC includes all LC and LC + 1PL species. HC includes all HC, HC + 1PL, HC + 2PL, and HC + 3PL species. (B) Light and heavy chain species with varied numbers of conjugated payload(s), each species includes all H₂O states. LC: light chain, HC: heavy chain, PL: payload, H₂O: thio-succinimide ring hydrolysis. Representative MS1 spectra of these species can be found in Figure S4.