FIG. 3.

Northern blot analysis of HIV-1 RNA expression in astrocytes infected with pseudotyped HIV-1. Astrocytes were infected with VSV/NL4-3, MLV/NL4-3, or NL4-3 and analyzed for HIV-1 RNA by Northern blotting 2, 14, and 28 days after infection as described in Materials and Methods. A total of 20 μg of total-cell RNA was loaded per lane. Samples from day 2 and 14 were exposed for 24 h, and samples from day 28 were exposed for 14 days. NL4-3-infected H9 cells were analyzed in parallel as positive controls; the samples were exposed for 24 h.