Figure 6.

Anti-PEG antibodies influence particle phagocytosis and CLL targeting in the blood of CLL patients. (a,b) Anti-PEG IgG levels in the plasma positively correlate with the phagocytosis of Doxil nanoparticles with and without BsAb (CD20 or CD28) functionalization by monocytes and granulocytes. (c) Anti-PEG IgM levels in the plasma negatively correlate with CLL targeting of CD20-functionalized PEG particles. Cell association (MFI) refers to the median fluorescence index of each cell type, stemming from fluorescence-labeled particles. The anti-PEG IgG and IgM levels in the plasma were determined by ELISA (Figure S11). Statistics were assessed by Spearman correlation analysis (n = 14).