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. 2005 Jun;138(2):654–662. doi: 10.1104/pp.104.052456

Figure 1.

Figure 1.

AtMAP65-1 and AtMAP65-6 cosediment with microtubules. A, Purification of AtMAP65-1 and AtMAP65-6. AtMAP65-1 fusion protein appeared in a doublet. AtMAP65-6 preparation had a minor band at 52 kD resulting from proteolysis. Positions of molecular mass markers were shown at right in kilodaltons. B, Cosedimentation of GST-MAP65-1 with microtubules. Cosedimentation experiments were performed with 0.5 and 5 μm fusion protein in the presence (+) or absence (−) of microtubules. Resulting proteins in the supernatants (S) and pellets (P) are shown. C, Cosedimentation of GST-MAP65-6 with microtubules. Similar to B, proteins in the supernatant (S) and pellets (P) were resolved by SDS-PAGE.