Microtubule bundles under depolymerization challenges. A, Microtubules totally depolymerized after cold treatment. B, Microtubule bundles induced by AtMAP65-1 were resistant to cold treatment. C, In the presence of AtMAP65-6, microtubules were not detected after cold treatment. D, Microtubules were depolymerized completely upon dilution to 50× with warm buffer in 60 min. E, Microtubule bundles induced by AtMAP65-1 were resistant to the dilution treatment. F, Microtubule network induced by AtMAP65-6 was sensitive to the dilution treatment as the network disappeared from the treatment. Scale bar = 10 μm.