Figure 6.

FACE analysis of leaf starch chain length distributions. Starch from leaves of wild-type, Atss3-1, and Atss3-2 plants was debranched with commercial isoamylase. The reducing ends of the linear chains were fluorescently labeled and separated by FACE. The frequency of individual chain lengths in each starch sample was normalized to total peak area. In the two bottom images, the normalized value for each chain length from the wild-type starch was subtracted from that of either the Atss3-1 or the Atss3-2 mutant. Values are the average of three independent determinations and se is indicated.