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Size fractionation of linear glucan chains. Starch from leaves of wild-type (solid line), Atss3-1 (dotted line), and Atss3-2 (dashed line) plants was debranched to completion with commercial isoamylase and the resulting linear chains were separated by GPC on a Sepharose CL-2B column. The Glc equivalents in each column fraction were quantified and plotted as a percentage of total Glc equivalents.
