Table 1. Parameters of Interest for ICT-Based Small-Molecule Fluorescent pH Probes.
| probes | MW | λex (nm)(pH) | λem (nm)(pH) | pKa | solution | model | refs |
|---|---|---|---|---|---|---|---|
| Z2 | 761 | 450 | 550 | 4.62 | PBS buffer | CCCP | (54) |
| HBTB2 | 644 | 436/566 | 612 | 8.04 | PBS buffer | H2O2 | (55) |
| PM-Mt-OH | 429 | 410/490 | 580/657 | PBS buffer with 0.1% DMSO | rapamycin | (56) | |
| CP | 279 | 400(4)/467(9) | 528(4)/606(9) | 5.88 | Britton-Robinson buffer | CCCP | (5) |
| HXPI-Cl | 460 | 694(8.5)/610(4.5) | 714(8.5)/678(4.5) | 5.77 | PBS buffer | rapamycin | (8) |
| NIR-HMA | 554 | 570 | 675(3)/710(8) | 6.50 | hypoxia | (57) | |
| SNRF | 439 | 510/560 | 586/628 | 8.3 | PBS buffer | rapamycin | (58) |
| M-pH | 354 | 435(3)/535(8) | 552(3)/584(8) | 6.87 | PBS buffer | sodium selenite/BSO | (59) |
| CEMT | 482 | 480 | 558(4.98)/620(9) | Britton-Robinson buffer | CE | (60) | |
| HBTMP | 488 | 375 | 470(8)/580(5) | 6.82 | Britton-Robinson buffer | CCCP | (61) |
| Mito-BNO | 620 | 405 | 468(8)/520(4) | 5.23 | PBS buffer | CQ/CCCP | (62) |
| ENBT | 368 | 428(4.42)/503(11.40) | 595(4.42)/700(11.40) | 7.98 | PBS buffer | CCCP | (63) |
| probe A-1 | 570 | 480 | 558(8)/698(3) | 8.26 | 30% ethanol | serum-free medium | (64) |
| BHC | 278 | 390/420 | 480 | 4.2/7.0 | PBS buffer | serum-free medium | (65) |
| HQO | 520 | 530(8.23)/710(6.59) | 650(8.23)/750(6.59) | 7.15 | 10 mM SDS in HEPES | liensinine/rapamycin | (1) |
| CM-BHNBD | 346 | 495 | 516 | 3.91 | Britton-Robinson buffer | oxidative stress | (66) |
| AH+ | 469 | 670 | 727 | 7.60 | citrate–phosphate buffer | FCCP | (67) |
| NIR-pH-H2O2 | 540 | 560 | 680 | 6.70 | PBS buffer | PMA | (68) |
| Cy-NH2 | 399 | 500 | 550(8)/670(3) | 3.85 | PBS buffer | serum-free media | (69) |