Table 2. Parameters of Interest for FRET-Based Small-Molecule Fluorescent pH Probes.
probe | MW | λex (nm)(pH) | λem (nm)(pH) | pKa | solution | model | refs |
---|---|---|---|---|---|---|---|
G-Mito | 402 | 405 | 550 | PBS buffer | H2O2/CQ | (27) | |
R-Lyso | 456 | 550 | 590 | PBS buffer | H2O2/CQ | (27) | |
FMOTY | 567 | 430 | 480 | Tris-HCl buffer | serum-free medium | (78) | |
AMTC | 624 | 550 | 590 | Tris-HCl buffer | serum-free medium | (78) | |
SCY-4 | 588 | 559 | 581 | PBS buffer | rapamycin | (79) | |
DMOTY | 586 | 405 | 510 | PBS buffer | rapamycin | (79) | |
RC-TPP | 1064 | 425/585 | 475/615 | Britton-Robinson buffer | CCCP | (80) | |
CRT | 1056 | 400(9)/560(3) | 475(9)/590(3) | DMSO | PMA | (81) | |
probe 8 | 620 | 405 | 472/576 | 6.96 | PBS buffer | H2O2 | (82) |
probe D | 682 | 436(3)/540(7) | 470(3)/660(7) | 6.01 | PBS buffer | (83) |