Figure 4.

Intracellular calcium response after stimulation with thapsigargin and CPA in hONPCs of HS and patients with SZ. Images were captured before stimulation (basal) and 2 min after stimulation with either 1 μM thapsigargin (TG, left graph) (A), and 10 μM cyclopiazonic acid (CPA, right graph) (B) in HS and SZ derived cells. No significant differences were found when basal Ca²⁺ levels were compared (HS vs. SZ), neither when comparing treated cell responses. Highly significant differences were found when basal Ca²⁺ levels were compared with their respective treatment. The graphs represent data obtained from at least six images (18–20 cells per image) per group. Data were expressed as mean ± SEM and compared using the Kruskal–Wallis test and Dunn’s multiple comparisons test, **** p < 0.0001. ns: not significant. Mean fluorescence intensity (MFI), arbitrary units (AU).