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. 2005 Jun;138(2):847–857. doi: 10.1104/pp.105.060517

Figure 2.

Figure 2.

Molecular characterization of ahk mutants. A, Northern analysis of expression of type cytokinin-induced genes in wild type (wt), ahk3, cre1, and the corresponding double mutant (cre1 ahk3). RNA was extracted from plants grown for 6 d and treated with or without kinetin (15 μm) for an additional day. Total RNA was isolated and RNA blots containing 15 μg of RNA per sample were subsequently hybridized to probes corresponding to the cytokinin-induced genes indicated. B, Influence of kinetin on the expression of Pi starvation-responsive genes in roots. Plants were grown in absence of Pi supplemented or not with 2 μm kinetin, and root material was collected after 9 d. Total RNA was isolated from roots, and RNA blots containing 15 μg of RNA per sample were subsequently hybridized to probes corresponding to the Pi starvation-responsive genes indicated.