Figure 3.

Time course of OPR2 expression in Arabidopsis seedlings treated with 0.313 mm RDX and 0.6 mm TNT. The analysis of gene expression is represented in the bar chart using average values of semiquantitative RT-PCR triplicate analysis. The results are represented with respect to the 0 h (control) fold induction, which was analyzed from nonexposed plants. Total RNA was extracted from three independent experiments for each chemical exposure. β-ATP internal standard is represented as the bottom band of each treatment. The top band represents OPR2 expression under different time exposures. Bars represent the means and sd of triplicate determinations.