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Enzymatic kinetic parameters of MsrB1 or MsrB2 by following the reduction of dabsyl-MetSO in the presence of DTE
KSulfoxide and kcat/KSulfoxide should be respectively divided and multiplied by two, considering that only the R isomer is a substrate of MsrB.
| KSulfoxide | kcat | kcat/KSulfoxide | |
|---|---|---|---|
| μm | s−1 | m−1s−1 | |
| B1 | 78.53 ± 2.17 | 0.07 ± 0.004 | 891 ± 78 |
| B2 | 54.06 ± 1.79 | 0.02 ± 0.002 | 370 ± 48 |
