FIG. 2.

Relative recovery of hypermutated sequences with normal and hypermutated PCR primers. Primers were designed to incorporate G-to-A substitutions in GA or GG dinucleotides (Materials and Methods) and compared to primers matched to normal sequences for their ability to amplify hypermutated sequences from the PBMC of 10 patients. A side-by side comparison of the PCR products using HA yellow gels is shown. N, normal primers; H, primers hyp and hypa. Hypermutated bands detected with primers hyp and hypa but not with normal primers are indicated for patients 3, 4, 5, 6, and 8 (asterisks). The positions of normal and fully hypermutated standards are indicated by arrows.