Accumulation of total SA in single and double mutants
SA and SA-glucoside levels determined by HPLC in the wild type, in single mutants and nahG, and in the acd11/eds5, acd11/sid2, and acd11/nahG double mutants as shown. “Young” refers to 18-d-old rosettes (third pair of true leaves emerging; all samples except “acd11/sid2 old”), and “old” refers to yellowing leaves on 34-d-old bolting plants. Each measurement is an average of triplicate samples, and the entire experiment was repeated twice with similar results.