Table I.

Purification of TPPII from Arabidopsis

	Total Protein		TPPII Activity		Purification	Yield
	Amount	Percentage	Totala	% Butabindide Sensitiveb
	mg	%	μmol L−1 min−1 mg−1	%	-fold	%
Crude extract	535	100	18	19	1	100
2% PEG	300	56	20	19	1	62
10% PEG	59	11	36	93	1	23
UnoQ6	7.6	1.4	44	98	12	17
Superose HR6	0.05	0.01	1,672	99	464	5

^aμmol L⁻¹ min⁻¹ mg⁻¹ AMC released using AAF-AMC as the substrate.

^bPercent total activity inhibited by 50 μm butabindide.