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. 2024 Oct 17;12(10):2371. doi: 10.3390/biomedicines12102371

Figure 6.

Figure 6

siRNA knockdown of LDHA alleviates OGD-induced M1 microglial polarization and inflammation. (A) LDHA knockdown significantly reduces lactate levels in BV2 cells post-OGD (n = 6). (B) Western blotting showing total protein lactylation and H3K9 lactylation levels in BV2 cells after LDHA knockdown (n = 3). (C) qPCR results showing reduced OGD-induced expression of M1 microglial markers Inos after LDHA knockdown (n = 6). (D) Western blotting showing reduced iNOS expression LDHA knockdown (n = 3). (E) qPCR results indicating reduced mRNA levels of pro-inflammatory genes after LDHA knockdown (n = 6). (F) LDHA knockdown significantly reduced the OGD-induced increase in TNFα (n = 3). Significance: * p < 0.05; ** p < 0.01; *** p < 0.001. ns, no significant difference.