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. 2005 Jun;138(2):1083–1096. doi: 10.1104/pp.104.058032

Figure 3.

Figure 3.

Cotransfection analysis of target gene specificities and cis-element requirements in At7 protoplasts. A, Cotransfection assays to determine the activation of the promoters of flavonoid biosynthesis genes by MYB12, ZmP, ZmSn, and the combination of ZmC1/ZmSn. The mean value of luciferase activities was 6,800 RLU μg−1 s−1. Samples were incubated for 20 h in the dark at 26°C prior to harvesting. B, Identification of cis-elements required for the activation of the CHS minimal promoter by MYB12, ZmP, and ZmC1+ZmSn. The mean value of luciferase activities was 7,600 RLU μg−1 s−1. Samples were incubated for 8 h in the dark at 26°C prior to harvesting. A and B, The reporter/effector combinations used are indicated. Columns represent the relative GUS′ activity in cotransfection experiments using discrete reporter/effector combinations. The highest occurring GUS′ activity measured for each individual reporter construct was set to 100%. Each column represents the mean of six independent measurements. Error bars illustrate the sd of mean values. The data tables below the graphs give the respective absolute GUS′ activity values measured. n.d., Not determined.